Mouse Subpooled CRISPRa-v2 Libraries-Membrane Proteins

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Mouse Subpooled CRISPRa-v2 Libraries-Membrane Proteins

Mouse Subpooled CRISPRa-v2 Libraries-Membrane Proteins

Catalog# LIBR-M005-P002 LIBR-M005-P100 LIBR-M005-P200 LIBR-M005-P500

Size 2ug 100ug 200ug 500ug

Instruction

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The CRISPR activation library targets 2,104 mouse menbrane protein genes with a total of 10,975 gene activation plasmid vectors, of which 5 different gRNA vectors designed for each gene, in addition to 250 control vectors targeting intergenic sequences. The library uses pCRISPRia-v2 as the backbone, which is a two plasmid system that expresses only gRNA, while the dCas9-VP64 gene is on the other vector that need to be used in combination.
Product Name
Mouse Subpooled CRISPRa-v2 Libraries-Membrane Proteins
Species
Mouse
Library Type
Activation Library
Plasmid System
Triple-plasmid System
Virus Packaging System
3rd Lentivirus Packaging System
Targeted Genes
2104
gRNA Number
10975
Non-targeting gRNA Number
250
Selection Marker
Puro ,tagBFP
Backbone Map
pCRISPRia-v2 vector formula
Click to view the full image
CRISPR iScreen™ Product Strength
  • 35+ Libraries
    100+ Cas9 cell lines for screening
    35+ Library types in stock, fulfilling different research needs Cas9 cells with high activity, good cell condition, easily accelerate CRISPR library construction.
    1
  • Plasmid
    Coverage>99%, uniformity<10
    The use of self-developed library specific competent cell makes it easier to capture exogenous DNA, with high transformation efficiency and low mutation risk.
    2
  • Cell Pool
    Coverage rate up to 99%
    Exclusive cell pool preparation process can achieve large-scale and standardized production of library cell pool, achieving fewer differences between batches and high repeatability.
    3
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